Polarimetry provides an additional check on a pure substance before it is added to an expensive batch to determine the ingredient’s concentration or purity for example a 25% glucose syrup will have an observed rotation that is five-sixths that of a 30% syrup. A polarimeter is a scientific instrument used to measure the angle of rotation caused by passing polarized light through an optically active substance some chemical substances are optically active, and polarized (uni-directional) light will rotate either to the left (counter-clockwise) or right (clockwise) when passed through these substances.
Choosing a polarimeter for pharmaceutical laboratories below is an example of why polarimeters with accuracy of ±002 and ±001 accuracy polarimeters are not suitable for pharmaceutical applications please review the usp monograph for the material ofloxacin. The autopol ii automatic polarimeter is a general-purpose polarimeter with ±001 degrees optical rotation accuracy this accuracy is applicable for food, university education, many chemical, flavor and fragrance applications, as well as pharmaceutical inspection in some countries.
Calibration of polarimeter short description to lay down the procedure for calibration of polarimeter sub category : quality control 51 operate the instrument as per the respective instrument sop and calibrate the polarimeter for linearity and accuracy from sucrose solutions of following concentration. Polarimeter of the m series are high resolution polarimeters with following features: accurate measuring of the optical activity of solutions according to icumsa, oiml, australian standard k157, european and american pharmacopeia. Quartz standards, sucrose solutions and optically active pure liquids are discussed as potential polarimetry standards it is suggested to preferably use quartz standards for checking the accuracy of perkin-elmer polarimeters quartz standards are absolutely stable, have a high accuracy and are easy to use.
Busy labs processing multiple samples per day now have the option of automated data capture, variable wavelength and temperature, and (with modern instruments such as the krüss p8000) up to 0002 deg accuracy and 0001 deg resolution. The polarimeter p3000 is the economical solution for standard applications for which a measurement accuracy of ±001° is sufficient it performs its tasks in the record measurement time of approx 1 s that you are familiar with from our digital polarimeters.
Calibrate a polarimeter for the accurate calibration of polarimeters, b+s recommend the use of a quartz control plate this has the following advantage s:-- t he value is reliable and traceable - a quartz plate is stable and provides a long term reference. For the most demanding high accuracy applications the autopol® vi automatic polarimeter technical bulletin 926 emmes survey the brand of polarimeter used in more us labs than any other.
The difference between these two models is their accuracy the polaar 30 polarimeter has an accuracy of ± 001 angular degrees, the polaar 3000 a higher accuracy of ± 0001 angular degrees both instruments have a zero, scale select and print keys.